Which of the following seperates proteins solely on the basis of it’s molecular size?
Correct Answer: Gel filtration chromatography
Description: Hydrophilic cross linked gels such as acrylamide, agarose and dextran are used for separation of molecules based on their size. Dextran ( sephadex) is widely used and the range of separation is based on pore size designated by the symbol G10 to G200. In this technique large molecules will come out of the column first and large molecules are retained in the column. This technique is used for separation of protein molecules, purification of proteins and molecular weight determination. Ref: Vasudevan Biochemistry, 3rd Edition, Page 56 ; Biotechnology 101 By Brian Shmaefsky, Page 89 ; Comprehensive Biotechnology XI By Dr. A. Jayakumaran Nair, Page 254
Category:
Biochemistry
Get More
Subject Mock Tests
Practice with over 200,000 questions from various medical subjects and improve your knowledge.
Attempt a mock test nowMock Exam
Take an exam with 100 random questions selected from all subjects to test your knowledge.
Coming SoonGet More
Subject Mock Tests
Try practicing mock tests with over 200,000 questions from various medical subjects.
Attempt a mock test now