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Skin
A 24 year old male presents to a STD clinic with a single painless ulcer on external genitalia. The choice of laboratory test to look for the etiological agent would be:
Scrappings from ulcer for culture on chocolate agar with antibiotic supplement.
Serology for detection of specific IgM antibodies.
Scrappings from ulcer for dark field microscopy.
Scrappings from ulcer for tissue culture.
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