Ophthalmology

Which of the following statement is true regarding Acanthamoeba keratitis?

For the isolation of the causative agent, corneal scraping should be cultured on a nutrient agar plate

The causative agent, Acanthamoeba is a helminth whose normal habitat is soil

Keratitis due to Acanthamoeba is not seen in the immunocompromised host

Acanthamoeba does not depend upon a human host for the completion of its life-cycle