400 mg/dL. "--Harrison 19/e p2446Friedewald EquationThe ultra centrifugal measurement of LDL is time consuming & expensive and require specialist equipment.For this reason, LDL-cholesterol is most commonly estimated from quantitative measurements of total and HDL-chntesterol and plasma triglycerides using the empirical relationship of Friedewald et al.Total cholesterol = LDL + HDL + Triglycerides/5Total cholesterol = LDL + HDL + Triglycerides/5300 = LDL+ 25+ 150/5LDL = 300 - (25 + 30) = 245 mg/dlLimitations of Friedewald EquationFriedewald Equation should not be used under the following circumstances:When chylomicrons are presentWhen plasma triglyceride concentration exceeds 400 mg/dLIn patients with dysbeta-lipoproteinemia (type III hyperlipoprteinemia)">