Gynaecology & Obstetrics Which one of the following biochemical parameters is the most sensitive to detect open spina bifida? A Maternal serum alpha fetoprotein. B Amniotic fluid alpha fetoprotein. C Amniotic fluid acetyl cholinesterase. D Amniotic fluid glucohexaminase. 💡 Explanation Which one of the following biochemical parameters is the most sensitive to detect open spina bifida? ✓ Correct Answer: B. Amniotic fluid alpha fetoprotein.