Medicine Which of the following represents the earliest manifestation of Cushing ‘s syndrome? A Loss of diurnal variation of coisol B Increased serum ACTH C Increased plasma Coisol D Increased urinary metabolites of Coisol 💡 Explanation Which of the following represents the earliest manifestation of Cushing 's syndrome? ✓ Correct Answer: A. Loss of diurnal variation of coisol