Biochemistry Which of the following reagents would be most useful in determining the N-terminal amino acid of a polypeptide? A Trypsin B Carboxypeptidase C Phenylisothiocyanate D Cyanogen bromide 💡 Explanation Which of the following reagents would be most useful in determining the N-terminal amino acid of a polypeptide? ✓ Correct Answer: C. Phenylisothiocyanate