Biochemistry Which of the following occurs during glycolysis? A Glucose is reduced to pyruvate in the cytosol of all cells. B The rate-limiting step is the formation of fructose-6-phosphate. C Glucose is phosphorylated by aldolase. D Pyruvate, NADH, and ATP are produced. 💡 Explanation Which of the following occurs during glycolysis? ✓ Correct Answer: D. Pyruvate, NADH, and ATP are produced.