Biochemistry Which of the following is the rate-limiting enzyme of gluconeogenesis? A Phosphofructokinase-1 B Pyruvate kinase C Glycerol kinase D Fructose 1,6 bisphosphatase 💡 Explanation Which of the following is the rate-limiting enzyme of gluconeogenesis? ✓ Correct Answer: D. Fructose 1,6 bisphosphatase