Biochemistry Which of the following is the rate-limiting enzyme of bile acid synthesis? A microsomal 7-a hydroxylase B mitochondrial 7-a hydroxylase C microsomal 17-a hydroxylase D mitochondrial 17-a hydroxylase 💡 Explanation Which of the following is the rate-limiting enzyme of bile acid synthesis? ✓ Correct Answer: A. microsomal 7-a hydroxylase