Biochemistry Which of the following is the rate limiting enzyme of bile acid synthesis A Mitochondrial 17 α hydroxylase B Cytoplasmic 7 α hydroxylase C Microsomal 7 α hydroxylase D Mitochondrial 7 α hydroxylase. 💡 Explanation Which of the following is the rate limiting enzyme of bile acid synthesis ✓ Correct Answer: C. Microsomal 7 α hydroxylase