Which of the following is the primary cell line –
Correct Answer: Chick embryo fibroblast
Description: Human diploid fibroblasts (HDF) cell line is used for cultivation of coxsackie A7,9,16; CMV; Echovirus;
enteroviruses; HSV-1,2; Poliovirus: parainfluenza virus, rhinovirus and VZV.
Cultivation of viruses
‘As viruses are obligate intracellular parasites they cannot be grown on any inanimate culture medium.' Three
methods are employed for cultivation of viruses :
1. Animal Inoculation
Earliest method
Mice are most widely employed animals
Other animals used are guinea pigs, rabbits and ferrets.
2. Embryonated eggs
Sites of inoculation are:
a) Chorioallantoic Membrane ------- visible pocks are produced → HSV, Vaccinia, Smallpox
b) Amniotic Sac
c) Allantoic cavity
→ Influenza
Some paramyxoviruses
d) Yolk Sac
→ Some viruses
Chlamydia
Rickettsia
3.Tissue culture
Most important method for virus isolation.
Three types of tissue culture are available
I. Organ Culture
II Explant Culture
III. Cell Culture - (routinely employed)
Cell culture may be
4. Primary cell culture Rhesus monkey kidney, human amnion, chick embryo fibroblast, human embryonic kidney.
5. Diploid cell strains:- Human diploid fibroblast HL-8, WI-38.
6. Continuous cell line:- Hela, HEP-2, McCoy, Vero.
Category:
Microbiology
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