Which of the following fungi is difficult to isolate on REGULAR SDA media

Correct Answer: Malassezia furfur
Description: M. furfur requires fatty acids for growth and does not grow on regular Sabouraud's medium Hence is cultured on the Sabouraud's dextrose agar (SDA) overlayed with a layer of olive oil. On incubation at 37degC, the fungus produces creamy colonies or Fried egg colonies within 5-7 days. Candida, Dermatophytes, Cryptococcus can be isolated easily on SDA.
Category: Microbiology
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