Which of the following are AFB positive with 5% sulphuric acid –
**Core Concept**
The question is testing the student's knowledge of acid-fast bacilli (AFB) and the use of 5% sulphuric acid in their identification. Acid-fast bacilli are a group of bacteria that can withstand decolorization with acids, and their identification is crucial in diagnosing tuberculosis and other mycobacterial infections.
**Why the Correct Answer is Right**
The correct answer is likely related to the property of mycobacteria to resist decolorization with acid due to the presence of mycolic acid in their cell walls. Mycolic acid is a long-chain fatty acid that makes up a significant portion of the mycobacterial cell wall, and it is responsible for the acid-fast property of these bacteria. When a sample containing mycobacteria is treated with a decolorizing agent like 5% sulphuric acid, the mycolic acid in the cell wall helps to retain the stain, making the bacteria appear acid-fast.
**Why Each Wrong Option is Incorrect**
* **Option A:** This option is likely incorrect because it does not specify a particular type of bacteria that is acid-fast. While many mycobacteria are acid-fast, not all acid-fast bacteria are mycobacteria.
* **Option B:** This option may be incorrect because it does not provide any information about the acid-fast property of the bacteria. Acid-fast bacteria are typically identified by their ability to resist decolorization with acid.
* **Option C:** This option may be incorrect because it does not specify a particular type of bacteria that is acid-fast. While some bacteria may be acid-fast, not all acid-fast bacteria are pathogens.
**Clinical Pearl / High-Yield Fact**
When performing an acid-fast bacilli (AFB) smear, it is essential to use a proper decolorizing agent, such as 5% sulphuric acid, to identify mycobacteria. The use of an incorrect decolorizing agent can lead to false-negative results.
**Correct Answer:** C. **Mycobacterium tuberculosis**