Which of the following agents are used to check sufficiency of a. Autoclaving b. Hydroclaving c. Microwaving
Correct Answer: a - Geobacillus stearothermophilus , b - Bacillus subtilis , c - Bacillus atrophaeus
Description: Thermal methods Principle Agent used to check its sufficiency 1. Autoclaving - Steam under high temperature - Temperature used in India = 121oC, >45 min ; 15 psi Geobacillus stearothermophilus 2. Hydroclaving - Steam under pressure - Temperature used = 121oC or 132oC Bacillus subtilis 3. Microwaving - Generation of convention current in heated water molecules - Frequency of 2450 MHz & wave length of 12.24 nm is used. Bacillus atrophaeus
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