Biochemistry Which is the only prokaryotic DNA polymerase with 5′-3′ exonuclease activity? A DNA Polymerase I B DNA Polymerase II C DNA Polymerase III D DNA Polymerase IV 💡 Explanation Which is the only prokaryotic DNA polymerase with 5'-3' exonuclease activity? ✓ Correct Answer: A. DNA Polymerase I