Physiology What effect does hyperventilation have on the oxygen binding affinity of hemoglobin? A P50 and oxygen affinity decrease B P50 and oxygen affinity increase C P50 decreases and the oxygen affinity increases D P50 increases and the oxygen affinity decreases 💡 Explanation What effect does hyperventilation have on the oxygen binding affinity of hemoglobin? ✓ Correct Answer: C. P50 decreases and the oxygen affinity increases