Viruses can be isolated from clinical samples by cultivation in the following except –
Correct Answer: Chemically defined media
Description: Cultivation of viruses
'As viruses are obligate intracellular parasites they cannot be grown on any inanimate culture medium'. Three methods are employed for cultivation of viruses :
1. Animal Inoculation
Earliest method
Mice are most widely employed animals
Other animals used are guinea pigs, rabbits and ferrets.
2. Embryonated eggs
Sites of inoculation are :
Chorioallantoic Membrane ----- visible pocks are produced → HSV, Vaccinia, Smallpox
Amniotic Sac
Allantoic Cavity
Influenza
Some paramyxoviruses
Yolk Sac
Some viruses
Chlamydia
Rickettsia
3. Tissue culture
Most important method for virus isolation.
Three types of tissue culture are available
Organ Culture
Explant Culture
Cell Culture - (routinely employed)
Category:
Microbiology
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