Tyndallization is a method of sterilization using;
Correct Answer: Moist heat at 100 C
Description: Tyndallization or intermittent sterilization uses moist heat at 100 C for 20 minutes for 3 consecutive days. It is carried out in a Koch and Arnold steam sterilizer. Media which contain any ingredient/component not able to tolerate the temperature of an autoclave are sterilized by tyndallization. Eg. TCBS, XLD, DCA, sugar solutions for fermentation tests and gelatin containing media.
Category:
Microbiology
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