Two plants grown in garden soil, one to express Growth Fluoro Protein (GFP) and other firefly luciferase. In dark, which of the following will be seen?
Correct Answer: Plant expressing firefly luciferase will glow
Description: Firefly luciferase is by far the most commonly used bioluminescent repoer. Firefly luciferase is slightly different from GFP, a photoprotein. Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to blue light. Photoproteins (GPF) don't require any special pigments, enzymes or chemicals to set it off. Once expressed, it simply needs mineral substrates to emit fluorescence. GFP was originally recruited from jellyfish. It has the same purpose as the luciferase enzyme in molecular biology, to mark the expression of select genes. At least in the lab, it GFP also requires to shine blue light (or sometimes UV) for it to emit the green fluorescence. It's such a standard tool now, in mice, plants, flies, fish studies.
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