True about autoclaving is: March 2007
## Core Concept
Autoclaving is a sterilization method that uses high-pressure steam to eliminate microorganisms from equipment and supplies. This process is critical in medical and laboratory settings to prevent infection and contamination. The effectiveness of autoclaving depends on factors such as temperature, pressure, and exposure time.
## Why the Correct Answer is Right
The correct answer, , implies that autoclaving involves the use of steam under pressure to achieve sterilization. This method is highly effective because steam penetrates materials easily, and the high temperature (usually 121°C) and pressure (15 psi) ensure that bacteria, viruses, and spores are killed. Autoclaving is widely used for sterilizing heat-resistant materials.
## Why Each Wrong Option is Incorrect
- **Option A:** Without the specific details of option A, it's not possible to directly address why it's incorrect. However, common misconceptions about autoclaving might include the idea that it uses dry heat or that it's only effective against certain types of microorganisms.
- **Option B:** Similarly, without the details of option B, we can't directly refute it. Generally, incorrect options might suggest that autoclaving is less effective, requires lower temperatures, or is used for materials that are not heat-resistant.
- **Option D:** Option D's incorrectness would depend on its specifics, but potential errors might include stating that autoclaving does not require pressure, uses a lower temperature, or is ineffective against spores.
## Clinical Pearl / High-Yield Fact
A crucial point to remember is that the standard parameters for autoclaving are a temperature of 121°C, a pressure of 15 psi, and a holding time of at least 15 minutes. This ensures that the process is lethal to all forms of microbial life, including bacterial spores, which are highly resistant to sterilization.
## Correct Answer Line
**Correct Answer: C.**