Biochemistry To initiate transcription RNA polymerase does not require which one of the following: A Template (ds DNA) B Activated precursors (ATP, GTP, UTP, CTP) C Divalent metal ions (Mn2+, Mg2+) D Primer 💡 Explanation To initiate transcription RNA polymerase does not require which one of the following: ✓ Correct Answer: D. Primer