The resolution provided by the light microscope is
**Core Concept**
The resolution of a light microscope refers to its ability to distinguish between two closely spaced points in a sample. This is limited by the wavelength of light used and the numerical aperture of the objective lens. The resolution is typically measured in micrometers (μm) and is a fundamental limitation in light microscopy.
**Why the Correct Answer is Right**
The resolution of a light microscope is determined by the diffraction limit, which is inversely proportional to the wavelength of light used. The shortest wavelength of visible light is approximately 400 nanometers (nm), which corresponds to a resolution of around 200-250 nm. However, the numerical aperture of the objective lens also plays a critical role in determining the resolution, with higher numerical apertures allowing for higher resolutions.
**Why Each Wrong Option is Incorrect**
**Option A:** This option is incorrect because it is too low, not accounting for the diffraction limit of visible light.
**Option B:** This option is incorrect because it is too high, exceeding the diffraction limit of visible light.
**Option C:** This option is incorrect because it is not a feasible resolution for a light microscope, given the diffraction limit of visible light.
**Clinical Pearl / High-Yield Fact**
The resolution of a light microscope is a fundamental limitation in microscopy, and understanding this concept is crucial in choosing the appropriate microscope for a given application.
**Correct Answer: C. 200-250 nm**