Biochemistry The reaction catalyzed by phosphofructokinase: A Is activated by high concentrations of ATP and citrate B Uses fructose-l-phosphate as substrate C Is the rate-limiting reaction of glycolytic Pathway? D Is inhibited by fructose 2, 6-bisphosphate 💡 Explanation The reaction catalyzed by phosphofructokinase: ✓ Correct Answer: C. Is the rate-limiting reaction of glycolytic Pathway?