Biochemistry The pKa values of primary and secondary phosphoryl groups of nucleotides are A 6.2 and 1.0 B 1.0 and 6.2 C 6.0 and 1.0 D 1.2 and 6.0 💡 Explanation The pKa values of primary and secondary phosphoryl groups of nucleotides are ✓ Correct Answer: B. 1.0 and 6.2