Microbiology The optimal time and temperature required for sterilization in an autoclave is A 140°C x 2 hrs. x 10 lbs pr B 121°F x 15 mts. x 15 lbs pr C 121°C x 15 sec. x 15 lbs pr D 121°C x 15 mts. x 15 lbs pr 💡 Explanation The optimal time and temperature required for sterilization in an autoclave is ✓ Correct Answer: D. 121°C x 15 mts. x 15 lbs pr