Biochemistry The major step of regulation of cholesterol synthesis is: A Cyclization of squalene to lanosterol B 3-hydroxy-3-methylglutaryl-CoA synthase C 3-hydroxy-3-methylglutaryl-CoA lyase D 3-hydroxy-3-methylglutaryl-CoA reductase 💡 Explanation The major step of regulation of cholesterol synthesis is: ✓ Correct Answer: D. 3-hydroxy-3-methylglutaryl-CoA reductase