Biochemistry The hydrolytic step leading to the release of a polypeptide chain from a ribosome is catalyzed by which of the following? A Dissociation of ribosomes B Peptidyl transferase C Release factors D Stop codons 💡 Explanation The hydrolytic step leading to the release of a polypeptide chain from a ribosome is catalyzed by which of the following? ✓ Correct Answer: B. Peptidyl transferase