The enzyme used in polymerase chain reaction (PCR) is:
Correct Answer: Taq polymerase
Description: Ans. A. Taq polymerasePolymerase Chain Reaction is a technique employed for DNA amplification. It requires the following apparatus:a. A target DNA.b. Two primers (synthetic oligonucleotides of 17-30 nucleotides length) that are complementary to flanked regions of DNA.c. Four dNTP's.d. A DNA polymerase mainly Taq DNA polymerase (Thermo stable). It has been isolated form Thermus aquaticus. It is heat resistant, so no need to add fresh enzyme in every cycle.
Category:
Biochemistry
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