The culture media used for cultivation of HSV is
**Core Concept**
The cultivation of Herpes Simplex Virus (HSV) requires a specific type of culture medium that supports the growth of the virus while inhibiting the growth of surrounding cells. This medium must have a balanced pH, adequate nutrients, and a defined growth environment.
**Why the Correct Answer is Right**
The correct culture medium used for HSV cultivation is Eagle's Minimum Essential Medium (EMEM) supplemented with 2% fetal bovine serum (FBS) and antibiotics. This medium provides the necessary nutrients, such as amino acids, vitamins, and glucose, for HSV to grow. The addition of FBS and antibiotics helps to support the growth of the virus while inhibiting the growth of surrounding cells.
**Why Each Wrong Option is Incorrect**
**Option A:** Sabouraud Dextrose Agar is a medium used for cultivating fungi, not viruses like HSV.
**Option B:** Luria Bertani (LB) agar is a medium used for cultivating bacteria, not viruses like HSV.
**Option C:** McCoy cells are a type of cell line used for cultivating viruses, but they are not a culture medium.
**Clinical Pearl / High-Yield Fact**
Remember that HSV can be grown in cell culture, but it requires a specific medium that supports its growth while inhibiting the growth of surrounding cells.
**Correct Answer: C. McCoy cells are not a culture medium but are used for cultivation of viruses in cell culture.**