Gynaecology & Obstetrics The best way of diagnosing Trisomy 21 during second trimester of pregnancy is: A Triple marker estimation B Nuchal skin fold thickness measurement C Chorionic villus sampling D Amniocentesis 💡 Explanation The best way of diagnosing Trisomy 21 during second trimester of pregnancy is: ✓ Correct Answer: D. Amniocentesis