In `Hotair oven’ for holding period of one hour, temperature required is –
**Core Concept**
The hot air oven is a type of sterilization equipment used to sterilize heat-resistant materials by subjecting them to dry heat. The holding period and temperature are critical parameters in the sterilization process to ensure effective sterilization.
**Why the Correct Answer is Right**
The hot air oven requires a temperature of at least 170°C for a holding period of 1 hour. This temperature is sufficient to kill all forms of microbial life, including bacteria, viruses, and spores. The dry heat from the oven penetrates the material, causing the proteins and nucleic acids to denature and die. The 1-hour holding period ensures that the material is exposed to the heat for an adequate duration to achieve sterilization.
**Why Each Wrong Option is Incorrect**
**Option A:** This option is incorrect because the temperature is too low to achieve effective sterilization. Most microorganisms require a temperature of at least 160°C to be killed.
**Option B:** This option is incorrect because the temperature is too high, which can cause damage to the material being sterilized.
**Option C:** This option is incorrect because the holding period is too short to ensure effective sterilization.
**Clinical Pearl / High-Yield Fact**
When using a hot air oven for sterilization, it's essential to ensure that the material being sterilized is compatible with the high temperatures and dry heat. Some materials, such as plastics or rubber, may degrade or become brittle when exposed to high temperatures.
**Correct Answer: C. 160°C**