In autoclave, the temperature is____ for a hold time of_____ at 15psi atmospheric pressure.
**Core Concept**
Autoclaving is a sterilization process that uses high pressure and temperature to kill microorganisms. The process involves heating the contents of a container in an autoclave chamber to a specific temperature and pressure, holding it for a certain period, and then cooling it down.
**Why the Correct Answer is Right**
The correct temperature for autoclaving is 121°C, which is the standard temperature used in many medical and laboratory settings. This temperature is achieved at a pressure of 15 psi (pounds per square inch) and is held for a specific period, typically 15-20 minutes, to ensure that all microorganisms are killed. The high pressure and temperature combination creates a steam environment that is lethal to bacteria, viruses, and other microorganisms.
**Why Each Wrong Option is Incorrect**
**Option A:** This option is incorrect because the temperature is too low to achieve effective sterilization. While 100°C is a high temperature, it is not sufficient to kill all microorganisms, especially spores.
**Option B:** This option is incorrect because the temperature is too high to be safely achieved in an autoclave. While 150°C is a high temperature, it is not a recommended temperature for autoclaving, as it can cause damage to certain materials.
**Option C:** This option is incorrect because the temperature is not the standard temperature used in autoclaving. While 110°C is a common temperature used in some autoclaving applications, it is not the standard temperature used in medical and laboratory settings.
**Clinical Pearl / High-Yield Fact**
It's essential to note that the temperature and pressure used in autoclaving can vary depending on the type of material being sterilized and the equipment being used. Always follow the manufacturer's instructions and guidelines for autoclaving to ensure effective sterilization and prevent damage to equipment.
**Correct Answer:** B. 121°C for a hold time of 15 minutes at 15psi atmospheric pressure.