**Core Concept**
The question tests the understanding of **glycolysis**, a metabolic pathway that converts glucose into pyruvate, releasing energy. **Substrate-level phosphorylation** is a type of metabolic reaction where a phosphate group is transferred from a high-energy compound to ADP to form ATP.

**Why the Correct Answer is Right**
In glycolysis, **phosphoglycerate kinase** and **pyruvate kinase** are the enzymes responsible for substrate-level phosphorylation. These enzymes catalyze the transfer of phosphate groups from high-energy intermediates to ADP, generating ATP.

**Why Each Wrong Option is Incorrect**
**Option A:** This option is incorrect because it does not match the known enzymes involved in substrate-level phosphorylation in glycolysis.
**Option B:** This option is incorrect because it does not match the known enzymes involved in substrate-level phosphorylation in glycolysis.
**Option D:** This option is incorrect because it does not match the known enzymes involved in substrate-level phosphorylation in glycolysis.

**Clinical Pearl / High-Yield Fact**
Remember that **phosphoglycerate kinase** and **pyruvate kinase** are key enzymes in glycolysis, catalyzing substrate-level phosphorylation reactions.

**Correct Answer:** Correct Answer: B. Phosphoglycerate Kinase and Pyruvate Kinase.