Biochemistry Substrate level phosphorylation in Glycolysis is seen in which step? A Glyceraldehyde 3 Phosphate dehydrogenase B Pyruvate kinase C Phosphofructokinase D Enolase 💡 Explanation Substrate level phosphorylation in Glycolysis is seen in which step? ✓ Correct Answer: B. Pyruvate kinase