Biochemistry Substitution of which one of the following amino acids in place of alanine would increase the absorbance of protein at 280 nm – A Leucine B Arginine C Tryptophan D Prolein 💡 Explanation Substitution of which one of the following amino acids in place of alanine would increase the absorbance of protein at 280 nm - ✓ Correct Answer: C. Tryptophan