Biochemistry Substitution of which one of the following amino acids in place of alanine would increase the absorbance of protein at 280 nm A Leucine B Arginine C Tryptophan D Proline 💡 Explanation Substitution of which one of the following amino acids in place of alanine would increase the absorbance of protein at 280 nm ✓ Correct Answer: C. Tryptophan