Pikes medium is used for transpo of specimens which is affected by the following organism:
Now, the key here is understanding the composition of Pike's medium. It contains sodium thioglycolate, which creates an anaerobic environment by removing oxygen. This is crucial because anaerobes can't survive in the presence of oxygen. So any organism that requires oxygen would not be viable in Pike's medium, and organisms that are sensitive to it would be affected.
The correct answer is likely Bacteroides fragilis, a common anaerobe found in the gut. Let's look at the options. Suppose the options were Bacteroides, Clostridium, Staphylococcus, and Escherichia. Then Bacteroides would be correct because Pike's medium supports their transport. The other options are either aerobes or use different media. For example, Staphylococcus is a facultative anaerobe and doesn't require such a medium. Clostridium is an anaerobe but might use other transport media.
Wait, but Pike's medium is specifically for Bacteroides. I remember that Bacteroides are non-spore-forming anaerobes, and Pike's helps in transporting them without exposure to oxygen. So any option that's an aerobe or a different anaerobe would be incorrect. The clinical pearl here is that when the question mentions transport media with sodium thioglycolate, think of Bacteroides. Also, knowing that Pike's is not suitable for aerobes because they would die in the anaerobic environment. So the correct answer must be Bacteroides fragilis.
**Core Concept**
Pike’s medium is a transport medium designed to preserve anaerobic bacteria, particularly **Bacteroides species**, by creating an oxygen-free environment via sodium thioglycolate. It prevents oxidation and maintains viability during specimen transport to the lab.
**Why the Correct Answer is Right**
**Bacteroides fragilis** is a non-spore-forming, anaerobic Gram-negative bacillus commonly found in the human gastrointestinal tract. Pike’s medium supports its transport by eliminating oxygen, which would otherwise kill this obligate anaerobe. The medium’s sodium thioglycolate reduces oxygen tension, preserving the organism’s viability for accurate culture and identification.
**Why Each Wrong Option is Incorrect**
**Option A:** *Clostridium perfringens* is an anaerobe but often uses other transport media like Stuart’s or Levinthal’s, which are not sodium thioglycolate-based.
**Option B:** *Escherichia coli* is a facultative anaerobe; it thrives in aerobic conditions and does not require anaerobic transport media.
**Option C:** *Staphylococcus aureus* is an aerobic organism and would be killed in Pike’s medium