In SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) proteins are separated on basis ofa) Massb) Chargec) Densityd) Molecular weighte) Solubility

## **Core Concept**
SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) is a technique used in molecular biology to separate proteins based on their size (molecular weight). The method involves denaturing proteins with SDS, a detergent that disrupts non-covalent bonds and coats proteins with a negative charge proportional to their length (or molecular weight).

## **Why the Correct Answer is Right**
The correct answer, **d) Molecular weight**, is right because in SDS-PAGE, SDS denatures proteins by wrapping around the polypeptide backbone, effectively giving each protein a uniform negative charge-to-mass ratio. As a result, when an electric field is applied, proteins migrate through the gel matrix at rates that are inversely proportional to their size (or molecular weight), allowing for their separation based on this property.

## **Why Each Wrong Option is Incorrect**
- **Option A: Mass** - While related, "mass" is not the specific term used in this context; "molecular weight" is more precise. However, it's not entirely incorrect but less specific than molecular weight.
- **Option B: Charge** - Charge is a basis for separation in other types of electrophoresis (e.g., native PAGE), but in SDS-PAGE, the charge is uniformized by SDS, making charge irrelevant for separation.
- **Option C: Density** - Density is not a factor in the separation mechanism of SDS-PAGE.
- **Option E: Solubility** - Solubility is important for the initial preparation of proteins for SDS-PAGE but is not the basis for their separation during the electrophoresis process.

## **Clinical Pearl / High-Yield Fact**
A key point to remember is that SDS-PAGE separates proteins based on size (molecular weight), which is crucial for identifying and quantifying proteins in a mixture. This technique is widely used in biochemistry and molecular biology for protein analysis.

## **Correct Answer: D. Molecular weight**