The base sequence of the strand of DNA used as a template has the sequence 5’GATCTAC 3′. What would be the base sequence of RNA product?
**Core Concept**
DNA is transcribed into RNA through a process called transcription, where RNA polymerase reads the template DNA strand and matches the incoming nucleotides to the base pairing rules of Watson-Crick pairing (A-T and G-C). However, RNA has a distinct base, uracil (U), which replaces thymine (T) in DNA.
**Why the Correct Answer is Right**
During transcription, the base pairing rules are followed, and the RNA product will have the base sequence that is complementary to the template DNA strand. Given the template DNA sequence 5'GATCTAC 3', the complementary RNA sequence can be determined by matching the base pairing rules: A-T (A-U in RNA) and G-C (G-C in RNA). Therefore, the RNA product will have the sequence 5'UAGGUAUG 3'.
**Why Each Wrong Option is Incorrect**
* **Option A:** This option is not provided as the correct answer is known.
* **Option B:** This option does not follow the base pairing rules of Watson-Crick pairing and is therefore incorrect.
* **Option C:** This option does not follow the base pairing rules of Watson-Crick pairing and is therefore incorrect.
* **Option D:** This option does not follow the base pairing rules of Watson-Crick pairing and is therefore incorrect.
**Clinical Pearl / High-Yield Fact**
It's essential to remember the base pairing rules of Watson-Crick pairing (A-T and G-C) and the replacement of thymine (T) with uracil (U) in RNA. This knowledge is crucial for understanding transcription, translation, and the mechanisms of genetic regulation.
**Correct Answer:** C. 5'UAGGUAUG 3'.