Selective plating medium for V. cholerae is:
**Core Concept**
Selective plating media are used to isolate specific types of microorganisms by inhibiting the growth of others. Thiosulfate-citrate-bile salts-sucrose (TCBS) agar is a selective medium specifically designed to support the growth of Vibrio species, including Vibrio cholerae, while inhibiting the growth of other bacteria.
**Why the Correct Answer is Right**
TCBS agar contains bile salts, which inhibit the growth of Gram-positive bacteria, and sucrose, which is fermented by Vibrio species. The thiosulfate and citrate components serve as energy sources for Vibrio growth. When Vibrio species ferment sucrose, they produce acid, which turns the agar yellow, making it easier to identify colonies. The selective properties of TCBS agar allow for the isolation of Vibrio cholerae from clinical samples.
**Why Each Wrong Option is Incorrect**
**Option A:** Carry-Blair medium is a transport medium used to preserve stool samples containing Vibrio cholerae during transport to the laboratory, but it is not a selective plating medium.
**Option C:** VR (Violet Red Bile Agar) medium is a selective medium used to isolate Enterobacteriaceae, particularly E. coli, and is not suitable for Vibrio species.
**Option D:** MacConkey agar is a selective medium used to differentiate lactose-fermenting from lactose-nonfermenting Gram-negative bacteria, and is not specific for Vibrio species.
**Clinical Pearl / High-Yield Fact**
Remember that TCBS agar is a selective medium specifically designed for Vibrio species, and its use can help prevent the overgrowth of other bacteria in stool samples.
β Correct Answer: B. TCBS agar