Rothera’s test used for detection of:
**Core Concept**
Rothera's test is a chemical test used for the detection of reducing substances, specifically glucose, in biological fluids. It is based on the reaction between glucose and nitroprusside, resulting in the formation of a purple-colored compound.
**Why the Correct Answer is Right**
The test relies on the reducing properties of glucose, which reacts with nitroprusside in the presence of a strong base to form a purple-colored compound. This reaction is specific to glucose and other reducing sugars. The mechanism involves the oxidation of the aldehyde group in glucose, leading to the formation of the characteristic purple color.
**Why Each Wrong Option is Incorrect**
**Option A:** is incorrect because Rothera's test is not used for detecting ketones.
**Option B:** is incorrect because the test is not specific for lactose or other disaccharides.
**Option C:** is incorrect because the test is not used for detecting amino acids.
**Clinical Pearl / High-Yield Fact**
Rothera's test is a classic example of a reducing sugar test, and it is often used in conjunction with Benedict's test to differentiate between reducing and non-reducing sugars.
**Correct Answer: C. Glucose.**