A group of elementary school-age children meet for a birthday party, and in the next few days, about half of them experience a mild upper respiratory illness, with sore throat and runny nose. One family gets laboratory work done and finds that Chlamydia pneumoniae (TWAR) is involved. Which of the following procedures would be best if the laboratory wanted to isolate this bacterium?

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Vikas Bhardwaj
Medical Technologist, AIIMS New Delhi
Updated: Apr 17, 2026