Reducing sugar in urine can be detected by ?
Correct Answer: All of the above
Description: Ans. is 'd' i.e., All of the aboveDetection of reducing sugars Reducing propey of sugars in alkaline solution is utilized for both qualitative and quantitative determination of sugars. Reagent containing Cu' ions are most commonly used. These are generally alkaline solution of cupric sulfate :-Benedict's quantitative reagent (CuSO4, Na2CO3, sodium citrate, potassium ferrocyanide, potassium thiocyanide) can detect any reducing sugar.Fehling solution contains CuSO4, Ruchelle Salt (sodium potassium taarate) and strong alkali (NaOH/ KOH). It is not used now.Glucose oxidase method : - This method is specific for glucose. Peroxidase and oxidase enzymes are used for estimation of glucose. These enzymes are the basis of highly specific test strips used for detction of glucose in urine or blood.
Category:
Biochemistry
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