Presence of which of the following in the expression of vector ensures increase in the yield of recombinant protein produced?
**Core Concept:** Recombinant protein production is a laboratory technique used in molecular biology to produce a protein of interest in a heterologous host organism, typically a bacteria or eukaryotic cell line. The expression of a recombinant plasmid ensures the production of the desired protein.
**Why the Correct Answer is Right:** Option C, "chloramphenicol resistance gene (cat gene)", is correct because it encodes for a protein that protects the bacteria from the toxic effects of chloramphenicol. When expressed, this gene leads to the production of the chloramphenicol acetyltransferase enzyme, which acetylates and inactivates chloramphenicol, preventing bacterial growth and allowing the recombinant protein to be produced without interference from the host cell proteins.
**Why Each Wrong Option is Incorrect:**
A. **Option A (lac Operon):** Although the lac operon is involved in the regulation of gene expression in E. coli, it is not directly responsible for recombinant protein production. The lac operon regulates the expression of genes encoding enzymes involved in lactose utilization, not recombinant protein production.
B. **Option B (T7 promoter):** The T7 promoter is a DNA region upstream of the T7 RNA polymerase gene that initiates transcription of the cloned gene into RNA. While it is essential for transcription and RNA synthesis, it does not directly contribute to recombinant protein production.
D. **Option D (tetracycline resistance gene):** The tetracycline resistance gene (tet gene) encodes for a protein that protects bacteria from the toxic effects of tetracycline. However, it does not directly contribute to recombinant protein production.
**Clinical Pearl:** The correct answer, option C, highlights the importance of proper gene regulation and selection markers in recombinant protein production techniques. This ensures that the recombinant protein is expressed without interference from the host cell proteins, allowing for efficient production and purification.
**Correct Answer:** Option C, "chloramphenicol resistance gene (cat gene)" ensures increased recombinant protein yield by providing selective pressure against the host organism while allowing the production of the desired recombinant protein without interference from endogenous host proteins.