Biochemistry Rate limiting step in vitamin D synthesis is? A 25 cholecalciferol B 1, 25-dihydroxycholecalciferol C 24, 25- dihydroxycholecalciferol D 7-dehydrocholesterol 💡 Explanation Rate limiting step in vitamin D synthesis is? ✓ Correct Answer: B. 1, 25-dihydroxycholecalciferol