For polymerase chain reaction which of the following is not required
**Core Concept**
Polymerase chain reaction (PCR) is a laboratory technique used to amplify specific DNA sequences. It involves the use of an enzyme called DNA polymerase, which is responsible for adding nucleotides to a growing DNA strand. The reaction requires a template DNA, primers, nucleotides, and a source of energy.
**Why the Correct Answer is Right**
Dideoxynucleotides are not required for PCR because they are used in sequencing reactions, such as the Sanger sequencing method, to terminate the elongation of DNA strands at specific points. In PCR, on the other hand, the goal is to amplify the DNA sequence, not to sequence it. The use of dideoxynucleotides would actually inhibit the PCR reaction by terminating the elongation of the DNA strand prematurely.
**Option A:** TAQ polymerase is required for PCR because it is the enzyme responsible for adding nucleotides to the growing DNA strand. TAQ polymerase is thermostable, meaning it can withstand the high temperatures required for the PCR cycling process.
**Option B:** d-NTP (deoxynucleoside triphosphates) are required for PCR because they provide the building blocks for the synthesis of new DNA strands. The d-NTPs are incorporated into the growing DNA strand by the TAQ polymerase enzyme.
**Option D:** Magnesium is required for PCR because it acts as a cofactor for the TAQ polymerase enzyme, facilitating the binding of the d-NTPs to the enzyme and the subsequent incorporation of the nucleotides into the growing DNA strand.
**Clinical Pearl / High-Yield Fact**
It's worth noting that PCR is a highly specific and sensitive technique that can be used to detect and quantify specific DNA sequences in a sample. This makes it a powerful tool in fields such as forensic science, genetic research, and diagnostic medicine.
**Correct Answer:**
β Correct Answer: C. Dideoxynucleotides