Which of the following is most common fixative used in electron microscope
## **Core Concept**
The core concept being tested here is the preparation of specimens for electron microscopy, specifically the fixation step. Fixation is a critical process that preserves the cellular structure, preventing degradation and maintaining the specimen's ultrastructural details.
## **Why the Correct Answer is Right**
The correct answer, **Glutaraldehyde**, is the most common fixative used in electron microscopy. Glutaraldehyde works by cross-linking proteins, effectively preserving the cellular structure. It is particularly useful for electron microscopy because it maintains the ultrastructural details of cells and tissues. Glutaraldehyde fixation is often followed by post-fixation with osmium tetroxide, which helps in preserving lipids and further enhancing the contrast of the specimen.
## **Why Each Wrong Option is Incorrect**
- **Option A:** Formaldehyde, while a common fixative in light microscopy, does not provide the same level of ultrastructural preservation as glutaraldehyde for electron microscopy.
- **Option B:** Ethanol is used in the dehydration process of specimen preparation for electron microscopy but is not a primary fixative.
- **Option D:** Acetone is also used in the dehydration process and in embedding procedures for electron microscopy but is not used as a primary fixative.
## **Clinical Pearl / High-Yield Fact**
A key point to remember is that for electron microscopy, the fixation step is critical and usually involves a combination of glutaraldehyde and osmium tetroxide. Glutaraldehyde is specifically noted for its ability to preserve the fine details of cellular structures.
## **Correct Answer:** . Glutaraldehyde