Biochemistry Michaelis Menten Hypothesis states that A Rate of enzymatic reaction is independent of substrate concentration B Rate of non enzymatic reaction is proportional to substrate concentration C Km is the enzyme substrate complex association constant D Enzyme substrate complex formation is essential in enzymatic reaction. 💡 Explanation Michaelis Menten Hypothesis states that ✓ Correct Answer: D. Enzyme substrate complex formation is essential in enzymatic reaction.