Method used for acid fast staining –
Correct Answer: Ziehl Neelsen
Description: Ans. is 'b' i.e., Ziehl Neelsen Acid fast stainingo After staining with aniline dye, acid fast organisms resist decolourisation with acids,o Method most commonly used is modified Ziehl Neelsen.Smear stained by carbolfuschin|Decolourization by 20% sulphuric acid|Counterstaining by methylene blueAcid fast organismsNon acid fast organismsRetain fiischin (red colour)Take colour of methylene blue (blue colour)Acid fastness depends uponMycolic acid Integrity of cell wall
Category:
Microbiology
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